CRBIP-Catalogue

Strain : CRBIP19.334 - Escherichia coli MFDpir

Identifier in the collection :

CRBIP19.334

Taxonomic name :

Escherichia coli MFDpir

Collection :

GGB

Type of microorganism :

Bacteria

Geographical origin :

Not applicable

Temperature :

37 °C

Atmosphere of incubation :

Aerobic

Bibliography :

J. Bacteriol, 2010, 192, 6418, doi:10.1128/JB.00621-10

Genotype :

MG1655 RP4-2-Tc::[ΔMu1::aac(3)IV-ΔaphA-Δnic35-ΔMu2::zeo] ΔdapA::(erm-pir) ΔrecA

GMO strain :

yes

History :

2013, JM. Ghigo, Institut Pasteur, Paris, France: strain MFDpir=UGB3093

Properties :

Mu-free donor strain for promoting mutagenic conjugation with RP4-dependent oriT plasmids Addition of 0.3 mM diaminopimelic acid (DAP Sigma D-1377) to the medium is strictly required for growth Resistances : apramycine (30 mg/ml), zeocine (50 mg/ml), erythromycine (200 mg/ml)

Catalogue comments :

MG1655 derivative Bachmann, B. (1996) Derivations and genotypes of some mutant derivatives of Escherichia coli K-12. In Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd edn. Neidhardt, F.C., Curtis, R., Ingraham, J.L., III, Lin, E.C., Low, K.B., and Magasanik, B. (eds). Washington, DC: American Society for Microbiology Press, pp. 2460-2488)

Packaging :

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