Formulation of medium : 10
MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria
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Columbia agar 39 g (
Bio-Rad
279240)
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Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
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Horse blood 100 ml (
BioMérieux
55 833)
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PolyVitex mischung 10 ml (
BioMérieux
55 651)
Dissolve agar at boiling Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically horse blood at 70°C during 15 minutes Cool to 50°C Add aseptically Polyvitex mischung
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