Formulation of medium : 101
MEDIUM 101 - for Kingella oralis
-
Tryptic soy agar 40 g (
BECTON DICKINSON
236950)
-
Bovin hemin 5 mg (
Sigma
H9039)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
-
Sheep blood 50 ml (
BioMérieux
55 823)
-
Sodium menadione 0.5 mg (
Sigma
M-2518)
Add all products except sheep blood and menadione Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically sheep blood and menadione
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