Formulation of medium : 1077
Luria Bertani Medium (cf medium 368) + Nalidixic Acid + Streptomycin + Rifampicin
-
Nalidixic Acid Solution at 30mg/ml 20 µl
-
Streptomycin Solution at 100mg/ml 20 µl
-
Rifampicin Solution at 100mg/ml 20 µl
Added with 20ml of Luria Bertani agar in fusion
Nalidixic Acid Solution at 30mg/ml 20 µl
-
Nalidixic Acid 300 mg (
SIGMA
N4382)
-
Distilled water make up to 10 ml
Filter sterilize
Distilled water make up to 10 ml
-
Ultra pure water make up to 10000 l (
Millipore
MilliQ)
NULL
Streptomycin Solution at 100mg/ml 20 µl
-
Streptomycin 1 g (
SIGMA
S6501)
-
Distilled water make up to 10 ml
Filter sterilize
Distilled water make up to 10 ml
-
Ultra pure water make up to 10000 l (
Millipore
MilliQ)
NULL
Rifampicin Solution at 100mg/ml 20 µl
-
Rifampicin 1 g (
SIGMA
R3501)
-
Distilled water make up to 10 ml
Filter sterilize
Distilled water make up to 10 ml
-
Ultra pure water make up to 10000 l (
Millipore
MilliQ)
NULL
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