Formulation of medium : 1082
Luria Bertani Medium (cf medium 368) + Tetracycline (7,5µg/ml final)
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Tetracycline Solution at 7,5mg/ml 20 µl
Added with 20ml of Luria Bertani agar in fusion
Tetracycline Solution at 7,5mg/ml 20 µl
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Tetracyclin 75 mg (
SIGMA
T3383)
Diluted in 10ml of 50% ethanol Filter sterilize
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