Formulation of medium : 1089
Luria Bertani Medium (cf medium 368) + Streptomycin + Chloramphenicol
-
Streptomycin Solution at 100mg/ml 20 µl
-
Chloramphenicol Solution at 25mg/ml 20 µl
Added with 20ml of Luria Bertani agar in fusion
Streptomycin Solution at 100mg/ml 20 µl
-
Streptomycin 1 g (
SIGMA
S6501)
-
Distilled water make up to 10 ml
Filter sterilize
Distilled water make up to 10 ml
-
Ultra pure water make up to 10000 l (
Millipore
MilliQ)
NULL
Chloramphenicol Solution at 25mg/ml 20 µl
-
Chloramphenicol USP 250 mg (
SIGMA
C0857)
Diluted in 10ml of 100% ethanol Filter sterilize
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