CRBIP-Catalogue

Formulation of medium : 1093

Luria Bertani Medium (cf medium 368) + Ampicillin + Streptomycin + Gentamycin

- Ampicillin Solution at 100mg/ml 20 µl
- Streptomycin Solution at 100mg/ml 20 µl
- Gentamycin solution at 10mg/ml 30 µl

Added with 20ml of Luria Bertani agar in fusion

Ampicillin Solution at 100mg/ml 20 µl

- Ampicillin sodium 1 g (^SIGMA A0166)

Diluted in 10ml of 50% ethanol Filter sterilize

Streptomycin Solution at 100mg/ml 20 µl

- Streptomycin 1 g (^SIGMA S6501)
- Distilled water make up to 10 ml

Filter sterilize

Distilled water make up to 10 ml

- Ultra pure water make up to 10000 l (^Millipore MilliQ)

NULL

Gentamycin solution at 10mg/ml 30 µl

- Gentamycin sulfate 100 mg (^SIGMA G3632)
- Distilled water make up to 10 ml

Filter sterilize

Distilled water make up to 10 ml

- Ultra pure water make up to 10000 l (^Millipore MilliQ)

NULL