Formulation of medium : 11
MEDIUM 11 - for Agromyces
-
Glucose 5 g (
Sigma
G8270)
-
Soytone bacto 5 g (
Becton Dickinson
211677)
-
Yeast extract 3 g (
BD Difco
212750)
-
Di Potassium monohydrogenophosphate 0.2 g (
VWR
26932.290)
-
Bacto Agar 15 g (
BD Difco
214010)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Add all products in boiling water except Di Potassium monohydrogenophosphate Cool to 50°C Add Di Potassium monohydrogenophosphate Adjust pH to 7.2 + 0.2 Autoclave at 110°C for 30 minutes
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