Formulation of medium : 110
MEDIUM 110 - for Halomonas meridiana
-
Sodium chloride 30 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium chloride hexahydrate 5 g (
Merck
1.05833)
-
Magnesium sulphate heptahydrate 9.5 g (
Merck
1.05886)
-
Potassium chloride 5 g (
Sigma
P9541)
-
Calcium chloride dihydrate 0.2 g (
Merck
102378)
-
Ammonium sulphate 0.1 g (
Merck
1211)
-
Potassium nitrate 0.1 g (
Prolabo
26 869.234)
-
Soytone bacto 5 g (
Becton Dickinson
211677)
-
Yeast extract 1 g (
BD Difco
212750)
-
Bacto Agar 15 g (
BD Difco
214010)
-
Ultra pure water make up to 960 ml (
Millipore
MilliQ)
-
Solution 2 - M00404 M00428 M00626 20.16 ml
-
Solution 3 - M0404 M0428 20.16 ml
-
Solution 4 - M0404 M0428 0.96 ml
Add all products except solutions Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically solutions
Solution 2 - M00404 M00428 M00626 20.16 ml
-
Nitrilotriacetic acid 10 g (
Sigma
N-9877)
-
Magnesium sulphate heptahydrate 29.7 g (
Merck
1.05886)
-
Calcium chloride dihydrate 3.3 g (
Merck
102378)
-
Sodium molybdate dihydrate 12.7 mg (
Merck
6521)
-
Iron (III) sulphate 99 mg (
Sigma
307718)
-
Metal 44 solution - M00634 M00736 50 ml
-
Ultra pure water make up to 950 ml (
Millipore
MilliQ)
Adjust pH to 7.2 + 0.2 Filter sterilize
Metal 44 solution - M00634 M00736 50 ml
-
Zinc sulphate heptahydrate 1.1 g (
Prolabo
29 158.237)
-
Iron (II) sulphate heptahydrate 0.5 g (
Prolabo
24 241.298)
-
E.D.T.A 0.25 g (
Sigma
E-5134)
-
Manganese II sulphate monohydrate 0.154 g (
Merck
1.05963)
-
Copper II sulphate pentahydrate 0.4 g (
Prolabo
23 174.290)
-
Cobalt II nitrate 0.025 g (
Prolabo
22.913)
-
Di-Sodium tetraborate anhydrous 0.018 g (
Merck
6309)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize
Solution 3 - M0404 M0428 20.16 ml
-
Di Potassium monohydrogenophosphate 2.5 g (
VWR
26932.290)
-
Potassium di-hydrogen phosphate 2.5 g (
Labosi
P1400)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes
Solution 4 - M0404 M0428 0.96 ml
-
Cyanocobalamin (Vit.B12) 0.1 mg (
Calbiochem
6791)
-
Biotin 2 mg (
Merck
1.24514)
-
Calcium D(+)- pantothenate 5 mg (
BDH
440744Y)
-
Folic acid 2 mg (
Prolabo
20 465.125)
-
Nicotinamide 5 mg (
VWR
481907.100)
-
Pyridoxal hydrochloride (vit B6) 10 mg (
BDH
440865Q)
-
Riboflavin (vit B2) 5 mg (
Sigma
R-4500)
-
Thiamine hydrochloride 5 mg (
Calbiochem
5871)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
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