Formulation of medium : 1102
Luria Bertani Medium (cf medium 368) + Ampicillin + Chloramphenicol
-
Ampicillin Solution at 100mg/ml 5 µl
-
Chloramphenicol Solution at 25mg/ml 40 µl
Added with 20ml of Luria Bertani agar in fusion
Ampicillin Solution at 100mg/ml 5 µl
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Ampicillin sodium 1 g (
SIGMA
A0166)
Diluted in 10ml of 50% ethanol Filter sterilize
Chloramphenicol Solution at 25mg/ml 40 µl
-
Chloramphenicol USP 250 mg (
SIGMA
C0857)
Diluted in 10ml of 100% ethanol Filter sterilize
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