Formulation of medium : 1103
Luria Bertani Medium (cf medium 368) + Spectinomycin (50µl/ml final)
-
Spectinomycin Solution at 25mg/ml 40 µl
Added with 20ml of Luria Bertani agar in fusion
Spectinomycin Solution at 25mg/ml 40 µl
-
Spectinomycin dihydrochloride 250 mg (
SIGMA
S9007)
-
Distilled water make up to 10 ml
Filter sterilize
Distilled water make up to 10 ml
-
Ultra pure water make up to 10000 l (
Millipore
MilliQ)
NULL
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