Formulation of medium : 1107
Luria Bertani Medium (cf medium 368) + Kanamycin (20µg/ml final)
-
Tryptone 10 g (
BECTON DICKINSON
211705)
-
Sodium chloride 10 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Yeast extract 5 g (
BD Difco
212750)
-
Bacto Agar 15 g (
BD Difco
214010)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
-
Kanamycin Solution at 25mg/ml 16 µl
Added with 20ml of Luria Bertani agar in fusion
Kanamycin Solution at 25mg/ml 16 µl
-
Kanamycin sulfate 250 mg (
SERVA
26899)
-
Distilled water make up to 10 ml
Filter sterilize
Distilled water make up to 10 ml
-
Ultra pure water make up to 10000 l (
Millipore
MilliQ)
NULL
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