Formulation of medium : 1111
Luria Bertani Medium (cf medium 368) + Chloramphenicol + Kanamycin + Zeocin + Tetracycline
-
Chloramphenicol Solution at 25mg/ml 20 µl
-
Kanamycin Solution at 25mg/ml 40 µl
-
Zeocin Solution at 100mg/ml 10 µl
-
Tetracycline Solution at 7,5mg/ml 20 µl
Added with 20ml of Luria Bertani agar in fusion
Chloramphenicol Solution at 25mg/ml 20 µl
-
Chloramphenicol USP 250 mg (
SIGMA
C0857)
Diluted in 10ml of 100% ethanol Filter sterilize
Kanamycin Solution at 25mg/ml 40 µl
-
Kanamycin sulfate 250 mg (
SERVA
26899)
-
Distilled water make up to 10 ml
Filter sterilize
Distilled water make up to 10 ml
-
Ultra pure water make up to 10000 l (
Millipore
MilliQ)
NULL
Tetracycline Solution at 7,5mg/ml 20 µl
-
Tetracyclin 75 mg (
SIGMA
T3383)
Diluted in 10ml of 50% ethanol Filter sterilize
Close
Processing
processing in progress, please wait...