CRBIP-Catalogue

Formulation of medium : 1114

Luria Bertani Medium (cf medium 368) + Tetracycline + Apramycin

- Apramycin Solution at 30mg/ml 20 µl
- Tetracycline Solution at 7,5mg/ml 20 µl

Added with 20ml of Luria Bertani agar in fusion

Apramycin Solution at 30mg/ml 20 µl

- Apramycin sulfate 300 mg (^SIGMA A2024)
- Distilled water make up to 10 ml

Filter sterilize

Distilled water make up to 10 ml

- Ultra pure water make up to 10000 l (^Millipore MilliQ)

NULL

Tetracycline Solution at 7,5mg/ml 20 µl

- Tetracyclin 75 mg (^SIGMA T3383)

Diluted in 10ml of 50% ethanol Filter sterilize