Formulation of medium : 1123
Luria Bertani Medium (cf medium 368) + Chloramphenicol + Zeocin
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Chloramphenicol Solution at 25mg/ml 20 µl
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Zeocin Solution at 100mg/ml 10 µl
Added with 20ml of Luria Bertani agar in fusion.
Chloramphenicol Solution at 25mg/ml 20 µl
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Chloramphenicol USP 250 mg (
SIGMA
C0857)
Diluted in 10ml of 100% ethanol Filter sterilize
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