Formulation of medium : 1128
Luria Bertani Medium (cf medium 368) + Kanamycin + Apramycin
-
Kanamycin Solution at 25mg/ml 40 µl
-
Apramycin Solution at 30mg/ml 20 µl
Added with 20ml of Luria Bertani agar in fusion
Kanamycin Solution at 25mg/ml 40 µl
-
Kanamycin sulfate 250 mg (
SERVA
26899)
-
Distilled water make up to 10 ml
Filter sterilize
Distilled water make up to 10 ml
-
Ultra pure water make up to 10000 l (
Millipore
MilliQ)
NULL
Apramycin Solution at 30mg/ml 20 µl
-
Apramycin sulfate 300 mg (
SIGMA
A2024)
-
Distilled water make up to 10 ml
Filter sterilize
Distilled water make up to 10 ml
-
Ultra pure water make up to 10000 l (
Millipore
MilliQ)
NULL
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