Formulation of medium : 1129
Luria Bertani Medium (cf medium 368) + Ampicillin + Tetracyclin
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Ampicillin Solution at 100mg/ml 20 µl
-
Tetracycline Solution at 7,5mg/ml 40 µl
Added with 20ml of Luria Bertani agar in fusion
Ampicillin Solution at 100mg/ml 20 µl
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Ampicillin sodium 1 g (
SIGMA
A0166)
Diluted in 10ml of 50% ethanol Filter sterilize
Tetracycline Solution at 7,5mg/ml 40 µl
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Tetracyclin 75 mg (
SIGMA
T3383)
Diluted in 10ml of 50% ethanol Filter sterilize
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