Formulation of medium : 131
MEDIUM 131 - for Blastochloris viridis
-
Yeast extract 1 g (
BD Difco
212750)
-
Potassium di-hydrogen phosphate 1 g (
Labosi
P1400)
-
Sodium carbonate hydrogen 1 g (
Merck
1.06329)
-
Ammonium sulphate 0.5 g (
Merck
1211)
-
Maleic acid 1 g (
Sigma
M-9009)
-
Solution SL-6 - M00545 1 ml
-
Bacto Agar 18 g (
BD Difco
214010)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 6.8 + 0.2 Autoclave at 121°C for 15 minutes.
Solution SL-6 - M00545 1 ml
-
Zinc sulphate heptahydrate 0.1 g (
Prolabo
29 158.237)
-
ManganeseII chloride tetrahydrate 0.03 g (
Prolabo
25 222.233)
-
Boric acid 0.3 g (
Prolabo
20 185.297)
-
Cobalt II chloride hexahydrate 0.2 g (
Sigma
C-2644)
-
Copper II chloride dihydrate 0.01 g (
Merck
2733)
-
Nickel II chloride hexahydrate 0.02 g (
Prolabo
25 850.290)
-
Sodium molybdate dihydrate 0.03 g (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
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