Formulation of medium : 137
MEDIUM 137 - for Ruminococcus hansenii and Rarobacter incanus
-
Tryptone 5 g (
BECTON DICKINSON
211705)
-
Soytone bacto 5 g (
Becton Dickinson
211677)
-
Yeast extract 10 g (
BD Difco
212750)
-
Beef extract 5 g (
Oxoïd
L29)
-
Glucose 5 g (
Sigma
G8270)
-
Di Potassium monohydrogenophosphate 2 g (
VWR
26932.290)
-
Tween 80 1 ml (
Merck
822187)
-
Resazurin sodium salt 1 mg (
Sigma
199303)
-
Salts solution - M00509 40 ml
-
L-Cystein 0.5 g (
Sigma
C7352)
-
Hemin solution - M00149 10 ml
-
Ultra pure water make up to 940 ml (
Millipore
MilliQ)
-
Vitamin solution - M00510 0.2 ml
Add all components except vitamin solution Adjust pH to 7.2 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically vitamin solution
Salts solution - M00509 40 ml
-
Calcium chloride dihydrate 0.25 g (
Merck
102378)
-
Magnesium sulphate heptahydrate 0.5 g (
Merck
1.05886)
-
Di Potassium monohydrogenophosphate 1 g (
VWR
26932.290)
-
Potassium di-hydrogen phosphate 1 g (
Labosi
P1400)
-
Sodium carbonate hydrogen 10 g (
Merck
1.06329)
-
Sodium chloride 2 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes.
Hemin solution - M00149 10 ml
-
Hemin chloride 0.1 g (
Merck
3741)
-
Sodium hydroxyde 30% 0.1 ml (
Fisher
S/4950/PB15)
-
Triethanolamine 4 ml (
VWR
28743.290)
-
Ultra pure water make up to 96 ml (
Millipore
MilliQ)
Filter sterilize
Vitamin solution - M00510 0.2 ml
-
Vitamin K1 0.1 ml (
Sigma
V-3501)
-
Ethanol 20 ml (
Merck
1.00983)
-
Ultra pure water make up to 1.3 ml (
Millipore
MilliQ)
Filter sterilize
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