CRBIP-Catalogue

Formulation of medium : 138

MEDIUM 138 - for Methylophaga thalassica and Methylophaga marina

- Sodium chloride 24 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Magnesium chloride hexahydrate 3 g (^Merck 1.05833)
- Magnesium sulphate heptahydrate 2 g (^Merck 1.05886)
- Potassium chloride 0.5 g (^Sigma P9541)
- Calcium chloride dihydrate 1 g (^Merck 102378)
- Trizma hydrochloride 0.5 g (^Sigma T-3253)
- Oligoelements - M0522 1 ml
- Ultra pure water make up to 972 ml (^Millipore MilliQ)
- Methanol 6 ml (^Merck 1.06018)
- Solution 2 - M0523 20 ml
- Vitamins solution - M0524 1 ml

Add all products except methanol, solution 2 and vitamins solution Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 30 minutes Add aseptically methanol, solution 2 and vitamins solution

Oligoelements - M0522 1 ml

- Strontium chloride hexahydrate 24 mg (^Sigma S-0390)
- Lithium chloride 17 mg (^Prolabo 25 009.236)
- Cobalt II chloride anhydrous 50 mg (^Merck 802540)
- Nickel II chloride hexahydrate 20 mg (^Prolabo 25 850.290)
- Copper II chloride dihydrate 10 mg (^Merck 2733)
- Sodium selenite 40 mg (^Prolabo 28.074)
- Manganese II sulphate monohydrate 40 mg (^Merck 1.05963)
- Zinc sulphate heptahydrate 50 mg (^Prolabo 29 158.237)
- Potassium iodure 60 mg (^Prolabo 26 843.235)
- Barium bromure dihydrate 60 mg (^Sigma B-8383)
- Sodium molybdate dihydrate 30 mg (^Merck 6521)
- Boric acid 30 mg (^Prolabo 20 185.297)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Autoclave at 110°C for 30 minutes

Solution 2 - M0523 20 ml

- Potassium di-hydrogen phosphate 15 g (^Labosi P1400)
- Ammonium chloride 100 g (^Sigma 09718)
- Ammonium iron (III) citrate 3 g (^Prolabo 24 145.230)
- Trizma hydrochloride 100 g (^Sigma T-3253)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH to 7.4 + 0.2 Autoclave at 110°C for 30 minutes

Vitamins solution - M0524 1 ml

- Cyanocobalamin (Vit.B12) 1 mg (^Calbiochem 6791)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Filter sterilize