CRBIP-Catalogue

Formulation of medium : 149

MEDIUM 149 - for Brachybacterium alimentarium and Brachybacterium tyrofermentans

- Tryptone 17 g (^{BECTON DICKINSON} 211705)
- Trypto casein soy broth 3 g (^Bio-Rad 64144)
- Yeast extract 5 g (^{BD Difco} 212750)
- Glucose 2.5 g (^Sigma G8270)
- Sodium chloride 5 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Di Potassium monohydrogenophosphate 2.5 g (^VWR 26932.290)
- Tween 80 1 ml (^Merck 822187)
- Bacto Agar 15 g (^{BD Difco} 214010)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 15 minutes