Formulation of medium : 164
MEDIUM 164 - for Flexibacter canadensis
-
Trizma hydrochloride 1.2 g (
Sigma
T-3253)
-
Casamino acids 1 g (
Becton Dickinson
223050)
-
Glucose 1 g (
Sigma
G8270)
-
Magnesium sulphate heptahydrate 0.1 g (
Merck
1.05886)
-
Potassium nitrate 0.1 g (
Prolabo
26 869.234)
-
Calcium chloride dihydrate 0.1 g (
Merck
102378)
-
Sodium glycerophosphate hexahydrate 0.1 g (
Prolabo
27 874.295)
-
ManganeseII chloride tetrahydrate 1.8 mg (
Prolabo
25 222.233)
-
Iron (II) sulphate heptahydrate 1.4 mg (
Prolabo
24 241.298)
-
Boric acid 2.8 mg (
Prolabo
20 185.297)
-
Zinc chloride 0.1 mg (
Merck
1.08816)
-
Sodium molybdate dihydrate 0.1 mg (
Merck
6521)
-
Bacto Agar 20 g (
BD Difco
214010)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
-
Vitamins solution - M00478 5 ml
Add all components except vitamins solution Adjust pH to 7.5 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically vitamins solution
Vitamins solution - M00478 5 ml
-
Biotin 0.1 g (
Merck
1.24514)
-
Cyanocobalamin (Vit.B12) 0.1 g (
Calbiochem
6791)
-
Thiamine hydrochloride 0.1 g (
Calbiochem
5871)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
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