CRBIP-Catalogue

Formulation of medium : 172

MEDIUM 172 - for Natronorubrum

- Di Potassium monohydrogenophosphate 1 g (^VWR 26932.290)
- Ammonium chloride 0.25 g (^Sigma 09718)
- Potassium chloride 0.5 g (^Sigma P9541)
- Magnesium sulphate heptahydrate 0.24 g (^Merck 1.05886)
- Calcium sulphate dihydrate 0.17 g (^Prolabo 22 452.237)
- Solution SL-10 - M00835 1 ml
- Bacto Agar 20 g (^{BD Difco} 214010)
- Sodium chloride 200 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Sodium glutamate 1 g (^Merck 6445)
- Yeast extract 5 g (^{BD Difco} 212750)
- Casamino acids 5 g (^{Becton Dickinson} 223050)
- Sodium carbonate solution - M00840 200 ml
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Add all components except SL-10 and sodium carbonate solutions Adjust pH to 9.2 + 0,2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically SL-10 and sodium carbonate solutions

Solution SL-10 - M00835 1 ml

- Hydrochloric acid 25% 10 ml (^Prolabo 20 257.296)
- Ferrous chloride tetrahydrate 1.5 g (^Merck 1.03861)
- Zinc chloride 70 mg (^Merck 1.08816)
- ManganeseII chloride tetrahydrate 100 mg (^Prolabo 25 222.233)
- Boric acid 6 mg (^Prolabo 20 185.297)
- Cobalt II chloride hexahydrate 190 mg (^Sigma C-2644)
- Copper II chloride dihydrate 2 mg (^Merck 2733)
- Nickel II chloride hexahydrate 24 mg (^Prolabo 25 850.290)
- Sodium molybdate dihydrate 36 mg (^Merck 6521)
- Ultra pure water make up to 990 ml (^Millipore MilliQ)

Sodium carbonate solution - M00840 200 ml

- Sodium carbonate anhydrous 5 g (^Prolabo 27 771.290)
- Ultra pure water make up to 200 ml (^Millipore MilliQ)

Autoclave at 121°C for 15 minutes.