CRBIP-Catalogue

Formulation of medium : 192

MEDIUM 192 - for Pseudoalteromonas rubra and Pseudomonas haloplanktis, Cytophaga lytica and Halomonas halodenitrificans

- Yeast extract 3 g (^{BD Difco} 212750)
- Soytone bacto 5 g (^{Becton Dickinson} 211677)
- Synthetic sea water solution - M00216 750 ml
- Bacto Agar 15 g (^{BD Difco} 214010)
- Ultra pure water make up to 250 ml (^Millipore MilliQ)

Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 15 minutes Used aged artificial sea water

Synthetic sea water solution - M00216 750 ml

- Sodium chloride 27.5 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Magnesium chloride hexahydrate 5 g (^Merck 1.05833)
- Magnesium sulphate heptahydrate 2 g (^Merck 1.05886)
- Calcium chloride dihydrate 0.5 g (^Merck 102378)
- Potassium chloride 1 g (^Sigma P9541)
- Iron (III) sulphate 1 mg (^Sigma 307718)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)