CRBIP-Catalogue

Formulation of medium : 198

MEDIUM 198 - for Haloarcula

- Sodium chloride 200 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Potassium chloride 4 g (^Sigma P9541)
- Magnesium sulphate heptahydrate 20 g (^Merck 1.05886)
- Magnesium chloride hexahydrate 13 g (^Merck 1.05833)
- Calcium chloride dihydrate 1 g (^Merck 102378)
- Sodium carbonate hydrogen 0.2 g (^Merck 1.06329)
- Potassium bromide 0.5 g (^Prolabo 26.704)
- Polypeptone 5 g (^{Becton Dickinson} 211910)
- Yeast extract 2 g (^{BD Difco} 212750)
- Bacto Agar 15 g (^{BD Difco} 214010)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH to 7.5 + 0.2 Autoclave at 121°C for 15 minutes