CRBIP-Catalogue

Formulation of medium : 2

MEDIUM 2 - for Acidomonas methanolica

- Proteose Peptone n°3 8 g (^Difco 211693)
- Yeast extract 1 g (^{BD Difco} 212750)
- Yeastolate 0.5 g (^{Becton Dickinson} 255772)
- Magnesium sulphate heptahydrate 0.2 g (^Merck 1.05886)
- Manganese II sulphate monohydrate 0.05 g (^Merck 1.05963)
- Sodium ammonium phosphate 2 g (^Merck 1.06682)
- Citric acid 0.6 g (^Sigma C-1056)
- Tween 80 1 ml (^Merck 822187)
- Glucose 15 g (^Sigma G8270)
- Maltose 5 g (^Merck 5910)
- Bacto Agar 20 g (^{BD Difco} 214010)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Dissolve agar at boiling Cool to 50°C Add all products Adjust pH to 5.0 + 0.2 Autoclave at 110°C for 30 minutes