Formulation of medium : 217
MEDIUM 217 - for Sporolactobacillus
-
Glucose 20 g (
Sigma
G8270)
-
Yeast extract 10 g (
BD Difco
212750)
-
Soytone bacto 10 g (
Becton Dickinson
211677)
-
Sodium acetate 10 g (
Prolabo
27 652.298)
-
Magnesium sulphate heptahydrate 2 g (
Merck
1.05886)
-
Iron (III) sulphate 0.1 g (
Sigma
307718)
-
Sodium chloride 0.1 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Manganese II sulphate monohydrate 0.16 g (
Merck
1.05963)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 6.8 + 0.2 Autoclave at 121°C for 15 minutes
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