Formulation of medium : 221
MEDIUM 221 - for Halorubrum lacusprofundi
-
Sodium chloride 180 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium chloride hexahydrate 75 g (
Merck
1.05833)
-
Magnesium sulphate heptahydrate 7.4 g (
Merck
1.05886)
-
Potassium chloride 7.4 g (
Sigma
P9541)
-
Calcium chloride dihydrate 1 g (
Merck
102378)
-
Disodium Succinate hexahydrate / Succinic acid disodium 10 g (
Merck
820151)
-
Yeast extract 1 g (
BD Difco
212750)
-
Ultra pure water make up to 990 ml (
Millipore
MilliQ)
-
Vitamins solution - M00478 10 ml
Add all components except vitamins solution Adjust pH to 7.4 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically vitamins solution
Vitamins solution - M00478 10 ml
-
Biotin 0.1 g (
Merck
1.24514)
-
Cyanocobalamin (Vit.B12) 0.1 g (
Calbiochem
6791)
-
Thiamine hydrochloride 0.1 g (
Calbiochem
5871)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
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