Formulation of medium : 223
MEDIUM 223 - for Halorubrum saccharovorum and H. distributum
-
Casamino acids 7.5 g (
Becton Dickinson
223050)
-
Yeast extract 10 g (
BD Difco
212750)
-
Tri-sodium citrate pentahydrate 3 g (
Prolabo
27 834.29)
-
Potassium chloride 2 g (
Sigma
P9541)
-
Magnesium sulphate heptahydrate 20 g (
Merck
1.05886)
-
Sodium chloride 250 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Iron (II) sulphate heptahydrate 0.05 g (
Prolabo
24 241.298)
-
Manganese II sulphate monohydrate 0.2 mg (
Merck
1.05963)
-
Bacto Agar 20 g (
BD Difco
214010)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 7.4 + 0.2 Autoclave at 121°C for 15 minutes
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