Formulation of medium : 225
MEDIUM 225 - for Natronococcus, Amylolyticus and Natronobacterium gregoryi
-
Potassium di-hydrogen phosphate 1 g (
Labosi
P1400)
-
Potassium chloride 1 g (
Sigma
P9541)
-
Ammonium chloride 1 g (
Sigma
09718)
-
Magnesium sulphate heptahydrate 0.24 g (
Merck
1.05886)
-
Calcium sulphate dihydrate 0.17 g (
Prolabo
22 452.237)
-
Metal solution - M00942 1 ml
-
Bacto Agar 20 g (
BD Difco
214010)
-
Sodium chloride 200 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Sodium glutamate 1 g (
Merck
6445)
-
Yeast extract 5 g (
BD Difco
212750)
-
Casamino acids 5 g (
Becton Dickinson
223050)
-
Sodium carbonate anhydrous 5 g (
Prolabo
27 771.290)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 9 + 0.2 Autoclave at 121°C for 15 minutes
Metal solution - M00942 1 ml
-
Zinc sulphate heptahydrate 0.1 g (
Prolabo
29 158.237)
-
ManganeseII chloride tetrahydrate 0.03 g (
Prolabo
25 222.233)
-
Boric acid 0.3 g (
Prolabo
20 185.297)
-
Cobalt II chloride hexahydrate 0.2 g (
Sigma
C-2644)
-
Copper II chloride dihydrate 0.01 g (
Merck
2733)
-
Nickel II chloride hexahydrate 0.02 g (
Prolabo
25 850.290)
-
Sodium molybdate dihydrate 0.03 g (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 3.0 + 0.2 Filter sterilize
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