CRBIP-Catalogue

Formulation of medium : 228

MEDIUM 228 - for Bacillus haloalkaliphilus

- Soytone bacto 5 g (^{Becton Dickinson} 211677)
- Bacto Agar 15 g (^{BD Difco} 214010)
- Ultra pure water make up to 900 ml (^Millipore MilliQ)
- Solution 1 - M00343 100 ml

Adjust pH to 9.7 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically solution 1

Solution 1 - M00343 100 ml

- Sodium carbonate hydrogen 4.2 g (^Merck 1.06329)
- Sodium carbonate decahydrate 5.3 g (^Merck 1.06391.1000)
- Ultra pure water make up to 100 ml (^Millipore MilliQ)