CRBIP-Catalogue

Formulation of medium : 249

MEDIUM 249 - for Thiobacillus

- Ammonium sulphate 0.1 g (^Merck 1211)
- Di Potassium monohydrogenophosphate 4 g (^VWR 26932.290)
- Potassium di-hydrogen phosphate 4 g (^Labosi P1400)
- Magnesium sulphate heptahydrate 0.1 g (^Merck 1.05886)
- Calcium chloride dihydrate 0.1 g (^Merck 102378)
- Ferric III chloride hexahydrate 2 mg (^Prolabo 24 208.237)
- Manganese II sulphate monohydrate 2 mg (^Merck 1.05963)
- Sodium thiosulphate pentahydrate 10 g (^Merck 6516)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH to 6.6 + 0.2 Autoclave at 115°C for 20 minutes