Formulation of medium : 253
MEDIUM 253 - for Streptococcus, Gemella haemolysans
-
Brain heart infusion 52 g (
BECTON DICKINSON
237500)
-
Glucose 10 g (
Sigma
G8270)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Add all products except horse serum Adjust pH to 7.4 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically horse serum
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