Formulation of medium : 26
MEDIUM 26 - for Gardnerella and Propionibacterium lymphophilum
-
Brain heart infusion 37 g (
BECTON DICKINSON
237500)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
-
Horse serum 200 ml (
Gibco
26050-088)
-
Maltose 10 % solution - M0173 200 ml
Adjust pH to 7.4 + 0.2 Add all products except horse serum and maltose solution Autoclave at 121°C for 15 minutes Add asepticallly horse serum and maltose 10 % solution
Maltose 10 % solution - M0173 200 ml
-
Maltose 20 g (
Merck
5910)
-
Ultra pure water make up to 200 ml (
Millipore
MilliQ)
Filter sterilize
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