Formulation of medium : 262
MEDIUM 262 - Columbia agar with 30 % horse blood
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Columbia agar 39 g (
Bio-Rad
279240)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
-
Horse blood 300 ml (
BioMérieux
55 833)
Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 15 minutes Cool to 60°C Add aseptically horse blood
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