Formulation of medium : 27
MEDIUM 27 - for Capnocytophaga gingivalis and Abiotrophia genus
-
Brain heart infusion 37 g (
BECTON DICKINSON
237500)
-
Horse serum 200 ml (
Gibco
26050-088)
-
Maltose 10 % solution - M0173 200 ml
-
Cysteine hydrochloride 5 % solution - M0172 111 ml
-
Ultra pure water make up to 489 ml (
Millipore
MilliQ)
Adjust pH to 7.4 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically horse serum and solutions
Maltose 10 % solution - M0173 200 ml
-
Maltose 20 g (
Merck
5910)
-
Ultra pure water make up to 200 ml (
Millipore
MilliQ)
Filter sterilize
Cysteine hydrochloride 5 % solution - M0172 111 ml
-
L-Cystein 10 g (
Sigma
C7352)
-
Ultra pure water make up to 200 ml (
Millipore
MilliQ)
Filter sterilize
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