CRBIP-Catalogue

Formulation of medium : 275

MEDIUM 275 - for Actinomadura rugatobispora and Actinomadura verrucosospora

- Starch maize 10 g (^Prolabo 21 145.296)
- Magnesium sulphate heptahydrate 1 g (^Merck 1.05886)
- Sodium chloride 1 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Ammonium sulphate 2 g (^Merck 1211)
- Calcium carbonate 2 g (^Prolabo 22 300.290)
- Bacto Agar 20 g (^{BD Difco} 214010)
- Trace salt solution - M00447 1 ml
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Add all components except trace salt solution Adjust pH to 7.2 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically trace salt solution

Trace salt solution - M00447 1 ml

- Iron (II) sulphate heptahydrate 0.1 g (^Prolabo 24 241.298)
- ManganeseII chloride tetrahydrate 0.1 g (^Prolabo 25 222.233)
- Zinc sulphate heptahydrate 0.1 g (^Prolabo 29 158.237)
- Ultra pure water make up to 100 ml (^Millipore MilliQ)

Filter sterilize