CRBIP-Catalogue

Formulation of medium : 282

MEDIUM 282 - for Ammoniphilus oxalaticus & Ammoniphilus oxalivorans

- Di-Ammonium oxalate 15 g (^Merck 1.01192)
- Sodium carbonate hydrogen 10 g (^Merck 1.06329)
- Di Sodium hydrogen phosphate 10 g (^Prolabo 28 026.292)
- Sodium chloride 0.7 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Potassium chloride 0.57 g (^Sigma P9541)
- Magnesium chloride hexahydrate 0.1 g (^Merck 1.05833)
- Calcium chloride dihydrate 0.01 g (^Merck 102378)
- Sodium thiosulphate pentahydrate 1 g (^Merck 6516)
- Bacto Agar 20 g (^{BD Difco} 214010)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust to pH 8.7 + 0.2 Autoclave at 121°C for 15 minutes