Formulation of medium : 289
MEDIUM 289 - for Acetobacter intermedius
-
Yeast extract 3 g (
BD Difco
212750)
-
Soytone bacto 4 g (
Becton Dickinson
211677)
-
Glucose 7.5 g (
Sigma
G8270)
-
Bacto Agar 8 g (
BD Difco
214010)
-
Ultra pure water make up to 940 ml (
Millipore
MilliQ)
-
Acetic acid 30 ml (
Prolabo
20 104.243)
-
Ethanol 30 ml (
Merck
1.00983)
Add all products except acetic acid and ethanol Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically acetic acid and ethanol
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