Formulation of medium : 294
MEDIUM 294 - for Pseudoalteromonas bacteriolytica
-
Casitone 1 g (
Becton Dickinson
225930)
-
Soytone peptone 1 g (
BECTON DICKINSON
243620)
-
Yeast extract 1 g (
BD Difco
212750)
-
Ammonium iron (III) citrate 0.2 g (
Prolabo
24 145.230)
-
Bacto Agar 15 g (
BD Difco
214010)
-
Ultra pure water make up to 250 ml (
Millipore
MilliQ)
-
Synthetic sea water solution - M00216 750 ml
Adjust pH to 7.5 + 0.2 Autoclave at 121°C for 15 minutes
Synthetic sea water solution - M00216 750 ml
-
Sodium chloride 27.5 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium chloride hexahydrate 5 g (
Merck
1.05833)
-
Magnesium sulphate heptahydrate 2 g (
Merck
1.05886)
-
Calcium chloride dihydrate 0.5 g (
Merck
102378)
-
Potassium chloride 1 g (
Sigma
P9541)
-
Iron (III) sulphate 1 mg (
Sigma
307718)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
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