CRBIP-Catalogue

Formulation of medium : 302

MEDIUM 302 - for Deinococcus geothermalis

- Yeast extract 2.5 g (^{BD Difco} 212750)
- Tryptone 2.5 g (^{BECTON DICKINSON} 211705)
- Nitrilotriacetic acid 0.1 g (^Sigma N-9877)
- Magnesium chloride hexahydrate 0.2 g (^Merck 1.05833)
- Ferric citrate 4.07 g (^Merck 3862)
- Mineral solution - M0552 0.5 ml
- Bacto Agar 28 g (^{BD Difco} 214010)
- Ultra pure water make up to 800 ml (^Millipore MilliQ)
- Phosphate buffer - M0553 100 ml
- Calcium sulphate solution - M0551 100 ml

Add all products except phosphate buffer and calcium sulphate solution Adjust pH to 6.5 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically phosphate buffer and calcium sulphate solution

Mineral solution - M0552 0.5 ml

- Nitrilotriacetic acid 12.8 g (^Sigma N-9877)
- Ferrous chloride tetrahydrate 1 g (^Merck 1.03861)
- ManganeseII chloride tetrahydrate 0.5 g (^Prolabo 25 222.233)
- Cobalt II chloride hexahydrate 0.3 g (^Sigma C-2644)
- Copper II chloride dihydrate 0.05 g (^Merck 2733)
- Sodium molybdate dihydrate 0.05 g (^Merck 6521)
- Boric acid 0.02 g (^Prolabo 20 185.297)
- Nickel II chloride hexahydrate 0.02 g (^Prolabo 25 850.290)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Filter sterilize

Phosphate buffer - M0553 100 ml

- Potassium di-hydrogen phosphate 5.44 g (^Labosi P1400)
- Di Sodium hydrogen phosphate 43 g (^Prolabo 28 026.292)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH to 7.2 + 0.2 Autoclave at 121°C for 15 minutes

Calcium sulphate solution - M0551 100 ml

- Calcium sulphate dihydrate 0.1 g (^Prolabo 22 452.237)
- Ultra pure water make up to 100 ml (^Millipore MilliQ)

Autoclave at 121°C for 15 minutes