Formulation of medium : 31
MEDIUM 31 - for Atopobium parvulum
-
Brain heart infusion agar 52 g (
BECTON DICKINSON
241830)
-
Tween 80 10 ml (
Merck
822187)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
-
Horse blood 100 ml (
BioMérieux
55 833)
Adjust pH to 7.4 + 0.2 Add all products except horse blood Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically horse blood
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