Formulation of medium : 313
MEDIUM 313 - for Alicyclobacillus acidoterrestris
-
Calcium chloride dihydrate 0.66 g (
Merck
102378)
-
Magnesium sulphate heptahydrate 0.18 g (
Merck
1.05886)
-
Ammonium sulphate 0.2 g (
Merck
1211)
-
Potassium di-hydrogen phosphate 3 g (
Labosi
P1400)
-
Yeast extract 1 g (
BD Difco
212750)
-
Glucose 5 g (
Sigma
G8270)
-
Mineral solution - M0611 1 ml
-
Ultra pure water make up to 500 ml (
Millipore
MilliQ)
-
Agar solution - M0614 500 ml
Add all products except agar solution Adjust pH to 4.0 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically agar solution
Mineral solution - M0611 1 ml
-
Calcium chloride dihydrate 0.66 g (
Merck
102378)
-
Zinc sulphate heptahydrate 0.18 g (
Prolabo
29 158.237)
-
Copper II sulphate pentahydrate 0.16 g (
Prolabo
23 174.290)
-
Manganese II sulphate monohydrate 0.15 g (
Merck
1.05963)
-
Cobalt II chloride hexahydrate 0.18 g (
Sigma
C-2644)
-
Boric acid 0.1 g (
Prolabo
20 185.297)
-
Sodium molybdate dihydrate 0.3 g (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
Agar solution - M0614 500 ml
-
Ultra pure water make up to 500 ml (
Millipore
MilliQ)
-
Bacto Agar 30 g (
BD Difco
214010)
Autoclave at 121° for 15 minutes
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