Formulation of medium : 317
MEDIUM 317 - for Alicyclobacillus acidocaldarius
-
Ammonium sulphate 1.3 g (
Merck
1211)
-
Potassium di-hydrogen phosphate 0.37 g (
Labosi
P1400)
-
Magnesium sulphate heptahydrate 0.25 g (
Merck
1.05886)
-
Calcium chloride dihydrate 0.07 g (
Merck
102378)
-
Ferric III chloride hexahydrate 0.02 g (
Prolabo
24 208.237)
-
Glucose 1 g (
Sigma
G8270)
-
Yeast extract 1 g (
BD Difco
212750)
-
Ultra pure water make up to 500 ml (
Millipore
MilliQ)
-
Agar solution - M0614 500 ml
Add all products except agar solution Adjust pH to 4.0 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically agar solution
Agar solution - M0614 500 ml
-
Ultra pure water make up to 500 ml (
Millipore
MilliQ)
-
Bacto Agar 30 g (
BD Difco
214010)
Autoclave at 121° for 15 minutes
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