Formulation of medium : 321
MEDIUM 321 - for Azospirillum lipoferum
-
Yeast extract 0.05 g (
BD Difco
212750)
-
Di Potassium monohydrogenophosphate 0.25 g (
VWR
26932.290)
-
Iron (II) sulphate heptahydrate 0.01 g (
Prolabo
24 241.298)
-
Sodium molybdate dihydrate 1 mg (
Merck
6521)
-
Manganese II sulphate monohydrate 2 mg (
Merck
1.05963)
-
Magnesium sulphate heptahydrate 0.2 g (
Merck
1.05886)
-
Sodium chloride 0.1 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Calcium chloride dihydrate 0.02 g (
Merck
102378)
-
Ammonium sulphate 1 g (
Merck
1211)
-
Bacto Agar 15 g (
BD Difco
214010)
-
Bromothymol blue 25 mg (
R.A.L
X93)
-
Ultra pure water make up to 950 ml (
Millipore
MilliQ)
-
Glucose solution - M0633 25 ml
-
Sodium malate 20 % solution - M0627 25 ml
Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes
Glucose solution - M0633 25 ml
-
Glucose 2.5 g (
Sigma
G8270)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize
Sodium malate 20 % solution - M0627 25 ml
-
Malic acid 20 g (
Sigma
M9138)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize
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