Formulation of medium : 330
MEDIUM 330 - for Natrinema pallidum
-
Yeast extract 5 g (
BD Difco
212750)
-
Casamino acids 5 g (
Becton Dickinson
223050)
-
Sodium glutamate 1 g (
Merck
6445)
-
Potassium chloride 2 g (
Sigma
P9541)
-
Citric acid 3 g (
Sigma
C-1056)
-
Magnesium sulphate heptahydrate 20 g (
Merck
1.05886)
-
Sodium chloride 200 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Ferrous chloride tetrahydrate 36 mg (
Merck
1.03861)
-
ManganeseII chloride tetrahydrate 0.36 mg (
Prolabo
25 222.233)
-
Bacto Agar 20 g (
BD Difco
214010)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes
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