Formulation of medium : 331
MEDIUM 331 -for Hymenobacter
-
Soytone bacto 0.25 g (
Becton Dickinson
211677)
-
Yeast extract 0.25 g (
BD Difco
212750)
-
Bacto Agar 15 g (
BD Difco
214010)
-
Ultra pure water make up to 965 ml (
Millipore
MilliQ)
-
Mineral solution - M00631 20 ml
-
Vitamins solution - M0632 5 ml
-
Glucose solution - M0633 10 ml
Add all products except solutions Adjust pH to 7.5 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically solutions
Mineral solution - M00631 20 ml
-
Magnesium sulphate heptahydrate 29.7 g (
Merck
1.05886)
-
Sodium molybdate dihydrate 12.67 g (
Merck
6521)
-
Nitrilotriacetic acid 10 g (
Sigma
N-9877)
-
Calcium chloride dihydrate 3.34 g (
Merck
102378)
-
Iron (II) sulphate heptahydrate 0.1 g (
Prolabo
24 241.298)
-
Metal 44 solution - M00634 M00736 50 ml
-
Ultra pure water make up to 950 ml (
Millipore
MilliQ)
Adjust pH to 7.2 + 0.2 Filter sterilize
Metal 44 solution - M00634 M00736 50 ml
-
Zinc sulphate heptahydrate 1.1 g (
Prolabo
29 158.237)
-
Iron (II) sulphate heptahydrate 0.5 g (
Prolabo
24 241.298)
-
E.D.T.A 0.25 g (
Sigma
E-5134)
-
Manganese II sulphate monohydrate 0.154 g (
Merck
1.05963)
-
Copper II sulphate pentahydrate 0.4 g (
Prolabo
23 174.290)
-
Cobalt II nitrate 0.025 g (
Prolabo
22.913)
-
Di-Sodium tetraborate anhydrous 0.018 g (
Merck
6309)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize
Vitamins solution - M0632 5 ml
-
Pyridoxal hydrochloride (vit B6) 0.02 g (
BDH
440865Q)
-
Benzoic acid 0.01 g (
Sigma
B-3375)
-
Calcium D(+)- pantothenate 0.01 g (
BDH
440744Y)
-
Nicotinic acid 0.01 g (
Calbiochem
481918)
-
Riboflavin (vit B2) 0.01 g (
Sigma
R-4500)
-
Thiamine hydrochloride 0.01 g (
Calbiochem
5871)
-
Biotin 4 mg (
Merck
1.24514)
-
Folic acid 4 mg (
Prolabo
20 465.125)
-
Cyanocobalamin (Vit.B12) 0.2 mg (
Calbiochem
6791)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
Glucose solution - M0633 10 ml
-
Glucose 2.5 g (
Sigma
G8270)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize
Close
Processing
processing in progress, please wait...