Formulation of medium : 345
MEDIUM 345 - for Polaribacter
-
Tryptone 0.5 g (
BECTON DICKINSON
211705)
-
Yeast extract 0.4 g (
BD Difco
212750)
-
Beef extract 0.4 g (
Oxoïd
L29)
-
Disodium Succinate hexahydrate / Succinic acid disodium 0.2 g (
Merck
820151)
-
Di Potassium monohydrogenophosphate 0.01 g (
VWR
26932.290)
-
Ammonium chloride 0.2 g (
Sigma
09718)
-
Synthetic sea solution - M0645 989 ml
-
Vitamins solution - M00646 (Balch,1979) 10 ml
-
Solution SL-6 - M00545 1 ml
Add all components except vitamins and SL-6 solutions Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically vitamins and SL-6 solutions
Synthetic sea solution - M0645 989 ml
-
Sodium chloride 24 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium sulphate heptahydrate 7 g (
Merck
1.05886)
-
Magnesium chloride hexahydrate 5.2 g (
Merck
1.05833)
-
Calcium chloride dihydrate 1.1 g (
Merck
102378)
-
Potassium chloride 0.7 g (
Sigma
P9541)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
Vitamins solution - M00646 (Balch,1979) 10 ml
-
Cyanocobalamin (Vit.B12) 0.1 mg (
Calbiochem
6791)
-
Biotin 2 mg (
Merck
1.24514)
-
Calcium D(+)- pantothenate 5 mg (
BDH
440744Y)
-
Folic acid 2 mg (
Prolabo
20 465.125)
-
Niacinamide (vit B3) 5 mg (
Sigma
N-5535)
-
Pyridoxal hydrochloride (vit B6) 10 mg (
BDH
440865Q)
-
Riboflavin (vit B2) 5 mg (
Sigma
R-4500)
-
Thiamine hydrochloride 5 mg (
Calbiochem
5871)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
Solution SL-6 - M00545 1 ml
-
Zinc sulphate heptahydrate 0.1 g (
Prolabo
29 158.237)
-
ManganeseII chloride tetrahydrate 0.03 g (
Prolabo
25 222.233)
-
Boric acid 0.3 g (
Prolabo
20 185.297)
-
Cobalt II chloride hexahydrate 0.2 g (
Sigma
C-2644)
-
Copper II chloride dihydrate 0.01 g (
Merck
2733)
-
Nickel II chloride hexahydrate 0.02 g (
Prolabo
25 850.290)
-
Sodium molybdate dihydrate 0.03 g (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
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