Formulation of medium : 35
MEDIUM 35 - for Flexibacter maritimus
-
Tryptone 0.5 g (
BECTON DICKINSON
211705)
-
Yeast extract 0.5 g (
BD Difco
212750)
-
Sodium acetate 0.2 g (
Prolabo
27 652.298)
-
Beef extract 0.2 g (
Oxoïd
L29)
-
Bacto Agar 10 g (
BD Difco
214010)
-
Synthetic sea water solution - M00216 700 ml
-
Ultra pure water make up to 300 ml (
Millipore
MilliQ)
Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 15 minutes
Synthetic sea water solution - M00216 700 ml
-
Sodium chloride 27.5 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium chloride hexahydrate 5 g (
Merck
1.05833)
-
Magnesium sulphate heptahydrate 2 g (
Merck
1.05886)
-
Calcium chloride dihydrate 0.5 g (
Merck
102378)
-
Potassium chloride 1 g (
Sigma
P9541)
-
Iron (III) sulphate 1 mg (
Sigma
307718)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
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