Formulation of medium : 358
MEDIUM 358 - for Pseudomonas vancouverensis, abietaniphila, multiresinivorans, resiniphila
-
Trypto casein soy broth 30 g (
Bio-Rad
64144)
-
Ultra pure water make up to 990 ml (
Millipore
MilliQ)
-
Pyruvic acid solution - M00704 10 ml
Add all components except Pyruvic acid solution Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically Pyruvic acid solution
Pyruvic acid solution - M00704 10 ml
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
-
Sodium Pyruvate 10 g (
Sigma
P-2256)
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